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polyclonal rabbit anti boil 17a  (Kingfisher Biotech)


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    Structured Review

    Kingfisher Biotech polyclonal rabbit anti boil 17a
    Polyclonal Rabbit Anti Boil 17a, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anti+boil+17a/Biotinylated+Anti-rabbit+IL-17A+PAb/pm33281817-79-101-104
    Average 91 stars, based on 2 article reviews
    polyclonal rabbit anti boil 17a - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Recombinant:

    Article Title: Hydrophobic Mycobacterial Antigens Elicit Polyfunctional T Cells in Mycobacterium bovis Immunized Cattle: Association With Protection Against Challenge?
    Article Snippet: IFN-γ was measured using the BOVIGAM ELISA (Prionics) according to the manufacturer’s instructions, with addition of a recombinant IFN-γ (ThermoFisher Scientific) standard curve. .. IL-17A was measured as described for bovine CXCL10 ( ) using polyclonal rabbit anti-boIL-17A (1.25 μg/ml), biotinylated polyclonal rabbit anti-boIL-17A (1.0 μg/ml), and recombinant boIL-17A (all from Kingfisher Biotech). .. IL-22 was measured by an in-house ELISA using recombinant bivalent anti-boIL-22 HuCAL antibodies ( ) and custom polyclonal anti-boIL-22 antibodies (Abcore).

    Article Title: Hydrophobic Mycobacterial Antigens Elicit Polyfunctional T Cells in Mycobacterium bovis Immunized Cattle: Association With Protection Against Challenge?
    Article Snippet: Concanavalin A (Sigma) was used at 2.5 μg/ml and PWM (Sigma) at 10 μg/ml. .. The following antibodies were used: anti-CD3 (MM1a), antiCD4 (ILA12), anti-CD8 (ILA51), anti-TCRd (GB21a), anti-CD25 (ILA111), anti-CD45-RO (ILA116), anti-CD172a (ILA24), antiDRB3 (ILA21), anti-DQ (CC158), anti-CD1b1/3 [bCD1b3 (33)], IgG2a isotype (Av37) all produced in-house; anti-CD25 (CACT108a), anti-CD4 (CC8), anti-DRB3 (TH14B), and antiDQ (TH81A5) from Washington State University Monoclonal Antibody Center; anti-CD4 (CC30), anti-CD14 (Tük4), polyclonal goat anti-huIgG F(ab’)2-FITC from Bio-Rad; antiCCR7 (3D12) from eBioscience; streptavidin-PE from Biolegend or BDPharmingen; anti-perforin (dG9) fromBDBiosciences; antigranulysin (rabbit polyclonal biotinylated IgG) from Raybiotech; rabbit polyclonal biotinylated IgG isotype from Abcam; mouse IgG1 and IgG2a isotype controls and polyclonal goat antimuIgG2b-AF633 from ThermoFisher Scientific; anti-boTCRd (GB21A) and (biotinylated) polyclonal rabbit anti-boIL-17A (Kingfisher Biotech); recombinant bivalent anti-boIL-22 Fab (34). .. For all flow cytometry experiments, antibodies were either directly conjugated with fluorochromes in house (Pacific BlueTM, Alexa FluorTM 488, and Alexa FluorTM 647 antibody labeling kit, Invitrogen), bought directly conjugated or labeled with isotypespecificfluorochrome-conjugatedantibodies.

    Article Title: Hydrophobic Mycobacterial Antigens Elicit Polyfunctional T Cells in Mycobacterium bovis Immunized Cattle: Association With Protection Against Challenge?
    Article Snippet: Concanavalin A (Sigma) was used at 2.5 μg/ml and PWM (Sigma) at 10 μg/ml. .. The following antibodies were used: anti-CD3 (MM1a), anti-CD4 (ILA12), anti-CD8 (ILA51), anti-TCRδ (GB21a), anti-CD25 (ILA111), anti-CD45-RO (ILA116), anti-CD172a (ILA24), anti-DRB3 (ILA21), anti-DQ (CC158), anti-CD1b1/3 [bCD1b3 ( )], IgG2a isotype (Av37) all produced in-house; anti-CD25 (CACT108a), anti-CD4 (CC8), anti-DRB3 (TH14B), and anti-DQ (TH81A5) from Washington State University Monoclonal Antibody Center; anti-CD4 (CC30), anti-CD14 (Tük4), polyclonal goat anti-huIgG F(ab’) 2 -FITC from Bio-Rad; anti-CCR7 (3D12) from eBioscience; streptavidin-PE from Biolegend or BD Pharmingen; anti-perforin (dG9) from BD Biosciences; anti-granulysin (rabbit polyclonal biotinylated IgG) from Raybiotech; rabbit polyclonal biotinylated IgG isotype from Abcam; mouse IgG1 and IgG2a isotype controls and polyclonal goat anti-muIgG2b-AF633 from ThermoFisher Scientific; anti-boTCRδ (GB21A) and (biotinylated) polyclonal rabbit anti-boIL-17A (Kingfisher Biotech); recombinant bivalent anti-boIL-22 Fab ( ). .. For all flow cytometry experiments, antibodies were either directly conjugated with fluorochromes in house (Pacific BlueTM, Alexa FluorTM 488, and Alexa FluorTM 647 antibody labeling kit, Invitrogen), bought directly conjugated or labeled with isotype-specific fluorochrome-conjugated antibodies.

    Produced:

    Article Title: Hydrophobic Mycobacterial Antigens Elicit Polyfunctional T Cells in Mycobacterium bovis Immunized Cattle: Association With Protection Against Challenge?
    Article Snippet: Concanavalin A (Sigma) was used at 2.5 μg/ml and PWM (Sigma) at 10 μg/ml. .. The following antibodies were used: anti-CD3 (MM1a), antiCD4 (ILA12), anti-CD8 (ILA51), anti-TCRd (GB21a), anti-CD25 (ILA111), anti-CD45-RO (ILA116), anti-CD172a (ILA24), antiDRB3 (ILA21), anti-DQ (CC158), anti-CD1b1/3 [bCD1b3 (33)], IgG2a isotype (Av37) all produced in-house; anti-CD25 (CACT108a), anti-CD4 (CC8), anti-DRB3 (TH14B), and antiDQ (TH81A5) from Washington State University Monoclonal Antibody Center; anti-CD4 (CC30), anti-CD14 (Tük4), polyclonal goat anti-huIgG F(ab’)2-FITC from Bio-Rad; antiCCR7 (3D12) from eBioscience; streptavidin-PE from Biolegend or BDPharmingen; anti-perforin (dG9) fromBDBiosciences; antigranulysin (rabbit polyclonal biotinylated IgG) from Raybiotech; rabbit polyclonal biotinylated IgG isotype from Abcam; mouse IgG1 and IgG2a isotype controls and polyclonal goat antimuIgG2b-AF633 from ThermoFisher Scientific; anti-boTCRd (GB21A) and (biotinylated) polyclonal rabbit anti-boIL-17A (Kingfisher Biotech); recombinant bivalent anti-boIL-22 Fab (34). .. For all flow cytometry experiments, antibodies were either directly conjugated with fluorochromes in house (Pacific BlueTM, Alexa FluorTM 488, and Alexa FluorTM 647 antibody labeling kit, Invitrogen), bought directly conjugated or labeled with isotypespecificfluorochrome-conjugatedantibodies.

    Article Title: Hydrophobic Mycobacterial Antigens Elicit Polyfunctional T Cells in Mycobacterium bovis Immunized Cattle: Association With Protection Against Challenge?
    Article Snippet: Concanavalin A (Sigma) was used at 2.5 μg/ml and PWM (Sigma) at 10 μg/ml. .. The following antibodies were used: anti-CD3 (MM1a), anti-CD4 (ILA12), anti-CD8 (ILA51), anti-TCRδ (GB21a), anti-CD25 (ILA111), anti-CD45-RO (ILA116), anti-CD172a (ILA24), anti-DRB3 (ILA21), anti-DQ (CC158), anti-CD1b1/3 [bCD1b3 ( )], IgG2a isotype (Av37) all produced in-house; anti-CD25 (CACT108a), anti-CD4 (CC8), anti-DRB3 (TH14B), and anti-DQ (TH81A5) from Washington State University Monoclonal Antibody Center; anti-CD4 (CC30), anti-CD14 (Tük4), polyclonal goat anti-huIgG F(ab’) 2 -FITC from Bio-Rad; anti-CCR7 (3D12) from eBioscience; streptavidin-PE from Biolegend or BD Pharmingen; anti-perforin (dG9) from BD Biosciences; anti-granulysin (rabbit polyclonal biotinylated IgG) from Raybiotech; rabbit polyclonal biotinylated IgG isotype from Abcam; mouse IgG1 and IgG2a isotype controls and polyclonal goat anti-muIgG2b-AF633 from ThermoFisher Scientific; anti-boTCRδ (GB21A) and (biotinylated) polyclonal rabbit anti-boIL-17A (Kingfisher Biotech); recombinant bivalent anti-boIL-22 Fab ( ). .. For all flow cytometry experiments, antibodies were either directly conjugated with fluorochromes in house (Pacific BlueTM, Alexa FluorTM 488, and Alexa FluorTM 647 antibody labeling kit, Invitrogen), bought directly conjugated or labeled with isotype-specific fluorochrome-conjugated antibodies.



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    Kingfisher Biotech polyclonal rabbit anti boil 17a
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    Kingfisher Biotech polyclonal rabbit anti boil 17a biotin
    Summary of flow cytometry antibody labelling panels <xref ref-type= a ." width="250" height="auto" />
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    Summary of flow cytometry antibody labelling panels <xref ref-type= a ." width="100%" height="100%">

    Journal: Scientific Reports

    Article Title: CD4+ and γδ T Cells are the main Producers of IL-22 and IL-17A in Lymphocytes from Mycobacterium bovis -infected Cattle

    doi: 10.1038/srep29990

    Figure Lengend Snippet: Summary of flow cytometry antibody labelling panels a .

    Article Snippet: Subsequently, cells were washed and surface labelled for 20 min. After a further wash cells labelled with unconjugated antibodies were incubated with a fluorochrome-labelled secondary antibody for 20 min. Before intracellular labelling (ICS), cells were washed and fixed with 1% formaldehyde (Cytofix, BD Biosciences, UK) over night at 4 °C, treated with Perm/Wash (BD Biosciences, UK) buffer according to manufacturer’s instruction and intracellularly labelled with recombinant bivalent boIL-22 Fab antibody, polyclonal rabbit anti-boIL-17A-biotin (Kingfisher Biotech, USA) and anti-boIFN-γ-PE (clone CC302, Bio-Rad AbD Serotec, UK) individually or in combination for 20 min followed by secondary labelling with polyclonal goat anti-huIgG F(ab’) 2 -FITC (Bio-Rad AbD Serotec, UK) and/or Streptavidin-fluorochrome for 20 min.

    Techniques: Flow Cytometry, Clone Assay, Recombinant